ʻElua Pūnaehana Pūnaehana Biosafety no ka hoʻāʻo wikiwiki antigen SARS-CoV-2
KA MANAO ANA
ʻO ka StrongStep®ʻO ka ho'āʻo Procalcitonin kahi wikiwiki immune-chromatographicassay no ka ʻike semi-quantitative o Procalcitonin i loko o ke kanaka serum a i ʻoleplasma.Hoʻohana ʻia ia no ka ʻike ʻana a me ka mālama ʻana i ka mālama ʻana i nā maʻi koʻikoʻi,ka maʻi bacteria a me ka sepsis.
OLELO HOOLAHA
ʻO ka Procalcitonin (PCT) kahi pūmua liʻiliʻi i loaʻa he 116 koena amino acidme ke kaumaha molekala ma kahi o 13 kDa i wehewehe mua iana Moullec et al.i ka makahiki 1984.Hana ʻia ʻo PCT ma nā ʻano C-cell o ka thyroid gland.I ka makahiki 1993, kakiʻekiʻe kiʻekiʻe o ka PCT i nā poʻe maʻi me kahi maʻi ʻōnaehana o ka kumu bacteriaua hōʻike ʻia a ua manaʻo ʻia ʻo PCT ka hōʻailona nui o nā maʻihele pū ʻia me ka mumū ʻōnaehana a me ka sepsis.Ka waiwai diagnostic oHe mea koʻikoʻi ka PCT ma muli o ka pilina pili ma waena o ka manaʻo PCT a meke kaumaha o ka mumū.Ua hōʻike ʻia ʻaʻole "inflammatory" PCThana ʻia i loko o nā pūnaʻi C.ʻO nā pūnaewele o ka neuroendocrine kumu paha ke kumuo ka PCT i ka wā o ka mumū.
KUMU
ʻO ka StrongStep®ʻIke ʻo Procalcitonin Rapid Test iā Procalcitonin ma o ka ʻike makaka wehewehe ʻana i ka hoʻomohala ʻana o ka waihoʻoluʻu ma ka ʻili o loko.ProcalcitoninHoʻopili ʻia ka antibody monoclonal ma ka ʻāpana hoʻāʻo o ka membrane.I ka wāka hoʻāʻo ʻana, pane ka specimen me nā antibodies anti-Procalcitonin monoclonalhoʻohui ʻia i nā ʻāpana kala a hoʻopaʻa ʻia ma luna o ka papa conjugate o ka hoʻāʻo.A laila neʻe ka hui ʻana ma o ka membrane e ka hana capillary ahui pū me nā reagents ma ka membrane.Inā lawa ka Procalcitonin i lokoʻo ka specimen, e hana ʻia kahi pūʻulu kala ma ka ʻāpana hoʻāʻo o ka membrane.ʻO kaʻO ka loaʻa ʻana o kēia pūʻulu kala e hōʻike ana i kahi hopena maikaʻi, ʻoiai ʻaʻole iahōʻike i kahi hopena maikaʻi ʻole.ʻO ke ʻano o kahi pūʻulu kala i ka mana'āina lawelawe ma ke 'ano he kaʻina hana, e hōʻike ana i ka leo kūpono oua hoʻohui ʻia ka specimen a ua hana ʻia ka membrane wicking.ʻO ka hoʻomohala ʻana o ka waihoʻoluʻu ʻokoʻa ma ka ʻāpana laina hoʻāʻo (T) hōʻike i kahi hopena maikaʻiʻoiai ka nui o Procalcitonin hiki ke loiloi semi-quantitative eka hoohalike ana o ka ikaika laina hoao i ka ikaika laina kuhikuhi ma kakāleka wehewehe.ʻO ka nele o kahi laina kala ma ka ʻāpana laina hoʻāʻo (T)hōʻike i kahi hopena maikaʻi ʻole.
MAHELE
No ka hoʻohana diagnostics IN VITRO wale nō kēia pahu.
■ E heluhelu pono i na kuhikuhi ma mua o ka hoao ana.
■ ʻAʻole i loaʻa i kēia huahana nā mea kumu kanaka.
■ Mai hoʻohana i nā mea i loko o ka pahu ma hope o ka lā pau.
■ E mālama i nā mea hoʻohālike a pau e like me ka maʻi maʻi.
■ E hahai i ke kaʻina hana Lab maʻamau a me nā kuhikuhina biosafety no ka lawelawe ʻana aka hoʻolei ʻana i nā mea hiki ke hoʻopaʻa ʻia.I ka wā e hana ʻia ai ke kaʻina hana assayhoʻopau, hoʻolei i nā mea hoʻohālike ma hope o ka autoclaving iā lākou ma 121 ℃ no ka liʻiliʻi loa20 min.ʻO kahi koho, hiki ke mālama ʻia me 0.5% Sodium Hypochloriteno nā hola ma mua o ka hoʻolei ʻana.
■ Mai pipette reagent ma ka waha a ʻaʻole puhi a ʻai ʻole i ka wā e hana ana i nā hoʻokolohua.
■ E hookomo i na lima lima i ka manawa o ka hana a pau.