Novel Coronavirus (SARS-CoV-2) Pūnaewele PCR Multiplex Real-Time

Hōʻike Pōkole:

ʻO ka coronavirus novel he virus RNA, i haku ʻia me nā protein a me nā waikawa nucleic. Hoʻokomo ka virus i ke kino o ka mea hoʻokipa (kanaka), komo i nā pūnaewele ma o ka paʻa ʻana o ka pūnaewele receptor ACE2, a kope i nā pūnaewele host, e pane ana i ka ʻōnaehana pale kino kanaka e pane i nā mea komo hewa haole a hana i nā antibodies kikoʻī. No laila, ʻo nā vic nucleic acid a me nā antigens, a me nā antibodies kikoʻī i ka coronavirus novel hiki ke hoʻohana ʻia ma ke ʻano he biomarkers kikoʻī no ka ʻike ʻana i ka coronavirus hou. No ka ʻike ʻana i ka waikawa nukelika, hoʻohana ʻia ka ʻenehana RT-PCR.

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit i manaʻo ʻia e hoʻohana ʻia e loaʻa ai ka ʻike kūpono o ka SARS_CoV-2 viral RNA i lawe ʻia mai nā swabs nasopharyngeal, swabs oropharyngeal, sputum a me BALF mai nā mea maʻi e pili pū me kahi FDA / CE ʻO ka ʻōnaehana hoʻoiho IVD a me nā paepae PCR i koho ʻia ma luna aʻe.

Hoʻonohonoho ʻia ka pahu e hoʻohana ʻia e nā limahana hoʻomaʻamaʻa Laboratory


ʻIke kikoʻī

Nā huahana huahana

Loaʻa i kēia kini PCR kitiki maʻalahi makaukau i ka format lyophilized (ke kaʻina maloʻo maloʻo) no ka mālama wā lōʻihi. Hiki ke lawe a mālama ʻia ka pahu i ka mahana o ka lumi a kūpaʻa ia no hoʻokahi makahiki. Loaʻa i kēlā me kēia paipu o ka premix nā reagents āpau e pono ai no ka hoʻonui PCR, e like me Reverse-transcriptase, Taq polymerase, primers, probes, a me nā substrates dNTPs. Pono wale ia e hoʻohui i ka wai i hoʻomaʻemaʻe 13ul a me 5ul i lawe ʻia i kahi anakuhi RNA, a laila hiki ke holo a hoʻonui ʻia i nā mea kani PCR.

Pono e hoʻokō ka mīkini qPCR i kēia mau koi:
1. Kūpono 8 kaomi PCR Tube nui 0.2 ml
2. Loaʻa ma mua o ʻehā mau kahawai ʻike:

Kanal

Hoʻoikaika (nm)

Ka hoʻokuʻu (nm)

Nā Leka Pre-Calibrated

1.

470

525

FAM, SYBR ʻōmaʻomaʻo I

2

523

564

VIC, HEX, TET, JOE

3.

571

621

ROX, TEXAS-ULAULA

4

630

670

CY5

Nā polokalamu PCR:
Pūnaewele PCR 7500Real-Time, Biorad CF96, iCycler iQ ™ Manaʻo Manawa Pūnaewele PCR, Stratagene Mx3000P, Mx3005P

ʻO ka paʻakikī o ka halihali kaulahao anu o Novel Coronavirus nukelika acid recectic reagent
Ke lawe ʻia nā reagents nukelea maʻamau i kahi mamao loa, koi ʻia ka (-20 ± 5) ℃ ke kaulahao anuanu a me ka halihali e hōʻoia i ka bioactive o ka enzyme i nā reagents e hoʻomau i ka hana. E hōʻoia i ka piʻi ʻana o ka mahana i ke ana, pono ʻia he mau kilokilo o ka hau maloʻo no kēlā me kēia pahu o ka hoʻāʻo ʻana o ka acidic acid ʻoi aku ka emi ma mua o 50g, akā hiki ke hala i ʻelua a ʻekolu paha lā. Ma ke kuanaʻike o ka hana ʻoihana, ke kaupaona maoli o nā mea hana i hoʻopuka ʻia e nā mea hana hana ma lalo o 10% (a i ʻole ʻoi aku ma lalo o kēia waiwai) o ka ipu. ʻO ka hapa nui o ke kaumaha mai ka hau maloʻo, nā ʻeke a me nā pahu pahū, no laila ke kiʻekiʻe loa ke kumu kūʻai.

Ma Malaki 2020, ua hoʻomaka ʻo COVID-19 e haki i kahi pae nui ma nā ʻāina ʻē, a ua piʻi nui ka noi no Novel Coronavirus nucleic acid detagent reagent. ʻOiai ke kumukūʻai kiʻekiʻe o ka lawe ʻana aku i nā reagents i ke kaulahao anuanu, hiki i ka hapanui o nā mea hana ke ʻae iā ia ma muli o ka nui a me ka loaʻa kālā kiʻekiʻe.

Eia nō naʻe, me ka hoʻomaikaʻi ʻana o nā kulekele kūwaho kūwaho no nā huahana anti-pandemik, a me ka hoʻomaikaʻi ʻana o ka kaohi aupuni i ke kahe o nā kānaka a me nā loina, aia ka hoʻolōʻihi a me ka maopopo ʻole i ka manawa halihali o nā mea reagents, a ʻo ia ka hopena o nā pilikia huahana i hoʻokumu ʻia. e ka halihali. Hoʻonui ʻia ka manawa halihali (ʻo ka manawa halihali ma kahi o ka hapalua mahina ka mea maʻamau) hiki i nā huahana huahana ʻole ke hiki ka huahana i ka mea kūʻai aku. Ua pilikia kēia i nā mea nui o nā mea hoʻoiho kūwaho.

Ua kōkua ka ʻenehana Lyophilized no ka PCR reagent i ka halihali ʻana o Novel Coronavirus i ka loaʻa ʻana o ka acidic acid i ke ao holoʻokoʻa.

Hiki ke lawe a mālama ʻia nā reagents PCR lyophilized a mālama ʻia i ka mahana wela, ʻaʻole hiki ke hoʻoliʻiliʻi wale i ke kumukūʻai o ka halihali, akā pale pū i nā pilikia maikaʻi i hoʻokumu ʻia e ke kaʻina halihali. No laila, ʻo ka lyophilizing o ka mea huli i ke ala ʻoi loa e hoʻonā i ka pilikia o ka lawe ʻana i ka lawe.

Hoʻopili ka Lyophilization i ke anuanu i kahi hopena i loko o kahi mokuʻāina paʻa, a laila sublimate a hoʻokaʻawale i ka mahu wai ma lalo o ke kūlana holo. Noho ka maloʻo maloʻo i loko o ka ipu me ka hana like a me ka hana. Hoʻohālikelike ʻia me nā reagents wai maʻamau, ʻo ka loiloi piha lyophilized Novel Coronavirus ʻike i ka mea loaʻa acidic acid i hana ʻia e Liming Bio i kēia mau ʻano:

ʻO ke kūpaʻa wela ikaika loa: hiki iā ia me ke kū ʻana i ka 56 ℃ no 60 mau lā, a me ka morphology a me ka hana o ka mea huli i koe hoʻololi ʻole.
Ka mālama ʻana i ka mahana maʻamau a me ka halihali: ʻaʻohe pono no ke kaulahao anuanu, ʻaʻole pono e mālama i ka mahana haʻahaʻa ma mua o ka hemo ʻana, e hoʻokuʻu piha i ka wahi anuanu.
Mākaukau e hoʻohana: lyophilizing o nā mea āpau, ʻaʻohe pono no ka hoʻonohonoho ʻōnaehana, pale i ka nalo ʻana o nā mea me ka viscosity kiʻekiʻe e like me ka enzyme.
Nā pahuhopu Multiplex i hoʻokahi pahu: uhi ka pahuhopu ʻike i ka genonavirus ORF1ab gene, ka ʻohana N, ka ʻaoʻao S e pale aku i ka genovariation virus. I mea e hōʻemi ai i ka maikaʻi ʻole wahaheʻe, hoʻohana ʻia ka gen RNase P kanaka e like me ka kaohi o loko, i mea e kō ai ka pono lapaʻau no ka mana o ka laʻana hoʻohālike.


  • Kekahi:
  • Aʻe:

  • Kākau i kāu leka ma aneʻi a hoʻouna iā mākou