Ho'āʻo wikiwiki ʻo Rotavirus Antigen
OLELO HOOLAHA
ʻO Rotavirus ka mea maʻamau maʻamau e pili ana i ka maʻi gastroenteritis, ka nui o nā keiki liʻiliʻi.ʻO kona loaʻa ʻana i ka makahiki 1973 a me kona hui pū ʻana me ka pēpē gastro-enteritis he mea nui loa ia i ke aʻo ʻana i ka gastroenteritis ʻaʻole i hoʻokumu ʻia e ka maʻi bacterial acute.Lawe ʻia ʻo Rotavirus ma ke ala waha-fecal me kahi manawa incubation o 1-3 mau lā.ʻOiai ʻo nā mea hoʻohālike i hōʻiliʻili ʻia i loko o ka lā ʻelua a me ka ʻelima o ka maʻi he mea kūpono no ka ʻike antigen, hiki ke loaʻa ka rotavirus i ka wā e hoʻomau ana ka maʻi maʻi.Hiki i ka Rotaviral gastroenteritis ke hopena i ka make no ka heluna kanaka i pilikia e like me nā kamaliʻi, nā ʻelemakule a me nā maʻi immunocompromised.Ma nā ʻano anuanu, loaʻa nā maʻi rotavirus i nā mahina hoʻoilo.Ua hōʻike ʻia nā maʻi maʻi a me nā maʻi ahulau e pili ana i kekahi mau tausani kānaka.Me nā keiki i hoʻopaʻa ʻia i ka haukapila e loaʻa ana i ka maʻi enteric acute, a hiki i ka 50% o nā specimen i hoʻopaʻa ʻia he maikaʻi no ka rotavirus.Hoʻopili hou nā virus i ka
nucleus cell a manaʻo e hoʻokipa i nā ʻano ʻano kikoʻī e hana ana i kahi hopena cytopathic hiʻohiʻona (CPE).No ka mea paʻakikī loa ka rotavirus i ka moʻomeheu, he mea maʻamau ka hoʻohana ʻana i ka hoʻokaʻawale ʻana o ka maʻi i ka ʻike ʻana i nā maʻi.Akā, ua hoʻomohala ʻia nā ʻano hana like ʻole e ʻike ai i ka rotavirus i nā feces.
KUMU
ʻIke ʻia ka Rotavirus Rapid Test Device (Feces) i ka rotavirus ma o ka wehewehe ʻike maka o ka hoʻomohala ʻana i ka waihoʻoluʻu ma ka ʻili o loko.Hoʻokuʻu ʻia nā antibodies anti-rotavirus ma ka ʻāpana hoʻāʻo o ka membrane.I ka hoʻāʻo ʻana, ka specimen
pane me nā anti-rotavirus antibodies i hoʻohui ʻia i nā ʻāpana kala a hoʻopaʻa mua ʻia ma luna o ka pahu hāpana o ka hoʻāʻo.A laila neʻe ka hui ʻana ma o ka membrane e ka hana capillary a hui pū me nā reagents ma ka membrane.Inā loaʻa
lawa ka rotavirus i loko o ka specimen, e hana ʻia kahi pūʻulu kala ma ka ʻāpana hoʻāʻo o ka membrane.ʻO ka loaʻa ʻana o kēia pūʻulu kala e hōʻike i kahi hopena maikaʻi, ʻoiai ʻo kona hele ʻole e hōʻike i kahi hopena maikaʻi ʻole.ʻO ke ʻano o kahi pūʻulu kala ma ka
ʻO ka ʻāina hoʻomalu ke ʻano he mana kaʻina hana, e hōʻike ana ua hoʻohui ʻia ka leo kūpono o ka specimen a ua hoʻokō ʻia ka membrane wicking.
NA KOMPONO KITS
| ʻO nā mea hoʻāʻo paʻa i hoʻopaʻa ʻia | Loaʻa i kēlā me kēia hāmeʻa kahi kaula me nā conjugates kala a me nā reagent reactive i uhi mua ʻia ma nā ʻāpana pili. |
| He paipu hoʻoheheʻe ʻia me ka buffer | 0.1 M Phosphate buffered saline (PBS) a me 0.02% sodium azide. |
| ʻO nā pipette hoʻopau | No ka ʻohi ʻana i nā ʻano wai |
| Hoʻokomo pūʻolo | No nā kuhikuhi hana |
MAKEMAKE IA NA MEA AKA AOLE I HANAIA
| Ka manawa | No ka hoʻohana manawa |
| Centrifuge | No ka mālama ʻana i nā specimens i nā kūlana kūikawā |
Nā palapala hōʻoia
